Helen C.Causton, Bing Ren, Sang Seok Koh, Christopher T.Harbison, Elenita Kanin, Ezra G.Jennings, Tong Ihn Lee, Heather L.True, Eric S.Lander, and Richard A.Young
Remodeling of Yeast Genome Expression in Response to Environmental Changes
Molecular Biology of the Cell Vol.12, 323-337,February 2001
[PDFダウンロード][Webサイト]
・Yeastの複数環境（刺激）下での遺伝子発現応答を、クラスタリング解析し、生物学的考察を加える。Gasch(2000)の論文とほぼ同じ構成。
・7種の環境（刺激）
1.Heat Shock [0 15 30 45 60 120 min]
2.Acid [10 20 40 60 80 100 min]
3.Alkali [10 20 40 60 80 100 min]
4.Hydrogen Peroxide(H2O2) [10 20 40 60 120 min]
5.Salt [15 30 45 60 120 min]
6.Sorbitol [15 30 45 90 120 min]
7.Diauxic Shift [9 11.5 13 15 16 18 20 h]
・クラスタリング：Eisen等によるソフトを使用。
・環境変化に応答する遺伝子を次の二つに分けて考察。
1.Common Environmental Response (CER) genes
2.Environmental-specific response genes
・問題点「The complete set of genes induced by various environmental changes has not been established; therefore, it is not yet clear that these activators are responsible for coordinate induction of these genes.」
・CER genesの定義「From this list, the genes that changed at least twofold in five or more time courses were selected as CER genes.」
・Environment-specific response genesの定義「To confer specificity, a second criterion required that genes induced in response to heat were not induced or repressed by more than two-fold in response to any other environment change (0.5＜fc＜2 for other time courses).」
・「The CER thus involves ～10% of the genome;」
・酵母遺伝子の性質「It is notable that many of the genes known to be induced in high-saline conditions, including ENA1, are induced by multiple stresses, suggesting that ion homeostasis is a critical response for most environmental changes.」
・わかったこと「We found that approximately two-thirds of the genome is involved in the response to environmental changes.」
・このデータは使えるかも。
《チェック》
・Winzeler EA et al,Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis., Science. 1999 Aug 6;285(5429):901-6.
Remodeling of Yeast Genome Expression in Response to Environmental Changes
Molecular Biology of the Cell Vol.12, 323-337,February 2001
[PDFダウンロード][Webサイト]
・Yeastの複数環境（刺激）下での遺伝子発現応答を、クラスタリング解析し、生物学的考察を加える。Gasch(2000)の論文とほぼ同じ構成。
・7種の環境（刺激）
1.Heat Shock [0 15 30 45 60 120 min]
2.Acid [10 20 40 60 80 100 min]
3.Alkali [10 20 40 60 80 100 min]
4.Hydrogen Peroxide(H2O2) [10 20 40 60 120 min]
5.Salt [15 30 45 60 120 min]
6.Sorbitol [15 30 45 90 120 min]
7.Diauxic Shift [9 11.5 13 15 16 18 20 h]
・クラスタリング：Eisen等によるソフトを使用。
・環境変化に応答する遺伝子を次の二つに分けて考察。
1.Common Environmental Response (CER) genes
2.Environmental-specific response genes
・問題点「The complete set of genes induced by various environmental changes has not been established; therefore, it is not yet clear that these activators are responsible for coordinate induction of these genes.」
・CER genesの定義「From this list, the genes that changed at least twofold in five or more time courses were selected as CER genes.」
・Environment-specific response genesの定義「To confer specificity, a second criterion required that genes induced in response to heat were not induced or repressed by more than two-fold in response to any other environment change (0.5＜fc＜2 for other time courses).」
・「The CER thus involves ～10% of the genome;」
・酵母遺伝子の性質「It is notable that many of the genes known to be induced in high-saline conditions, including ENA1, are induced by multiple stresses, suggesting that ion homeostasis is a critical response for most environmental changes.」
・わかったこと「We found that approximately two-thirds of the genome is involved in the response to environmental changes.」
・このデータは使えるかも。
《チェック》
・Winzeler EA et al,Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis., Science. 1999 Aug 6;285(5429):901-6.
アクセス
トータル
ランキング
